A multi-channel high speed data acquisition system is designed to collect acoustic emission signals of wood under long-term loading. Firstly, a four-channel acoustic emission signal hardware acquisition platform is built by using NI usb-6336 high-speed acquisition card, acoustic emission sensor and other hardware. Secondly, man-machine interface and software control system are designed based on Lab VIEW. Finally, the utility of the acquisition system is verified by wood three-point bending test. The experimental results show that the four-channel signal acquisition system can effectively collect and automatically store acoustic emission signals of wood during the damage process. As a kind of wood acoustic emission signal acquisition platform, this system can provide basic guarantee for the acquisition and analysis of wood acoustic emission signal.