This paper presented a multi-OD dynamic user equilibrium model based on the road network, in order to enable users to minimize travel time in the network. It uses Ant Colony algorithm to solve the problem of dynamic user equilibrium. By designing a numerical example and employing simulation we obtain traffic flow distribution data which compared traffic flow distribution data of Frank-Wolfe algorithm in the road network. The conclusion is that Ant Colony algorithm has certainly advantage in solving the problem of dynamic traffic user equilibrium.