This paper presents the studies and implementations of river and lake information system based on WebGIS technology in the Tarim river basin, which is concerned with the low efficiency of information acquisition of rivers and lakes in the Tarim river basin. The system mainly classifies the natural information, humanistic information, and spatial information of the rivers and lakes in the nine rivers in the Tarim river basin, Xinjiang, and realizes the display operation of the spatial data, the search and positioning of the rivers and lakes, multimodal query of river and lake information, rivers and lakes of the calculation, and other functions. The test results show that the system provides a convenient way to obtain information in the Tarim river basin, and has good practicability.