At present, the patrolling work of many natural reserves still uses the traditional patrolling way, which has seriously affected the efficiency of patrolling work. In this study, according to the needs of actual patrol work in natural reserves, the mobile patrol system of protected areas is constructed based on the main technologies of Android mobile development, Java Web development, KML, and LBS to replace the traditional patrolling way. The system includes the mobile terminal acquisition system and Web-side management system. Patrol staffs use the mobile terminal acquisition system to improve the efficiency of patrol information collection; protected area managers use the Web system to conduct efficient management of patrolling data, and timely learn the work of patrolling staff and real-time location. The system has been used in a multiple protected areas and has achieved good results.