For finding the ophthalmic diseases that can be observed from retinal vessels, fundus images play a key role and provide an effective reference for professional medical personnel. However, manual vessel segmentation has a large workload, which is time-consuming and laborious. Therefore, developing an automatic and intelligent segmentation method is of great benefit to relevant personnel. In this study, the attention mechanism and RU-Net structure are integrated into the generator of generative adversarial networks (GANs), forming a new structure—Retina-GAN. At the same time, automatic color equalization (ACE) is selected in the preprocessing of fundus images to improve image contrast and make blood vessels clearer. To validate the proposed approach, we compared the Retina-GAN with some other models on DRIVE datasets. Accuracy, sensitivity, and specificity are measured for comparative analysis. The experiment shows that Retina-GAN has better performance than other models.